Bioprocess cell perfusion system for biological process

Cell perfusion system

A cell perfusion system is a specialized setup used in cell culture and bioprocessing to provide a continuous flow of fresh media to a cell culture, allowing for extended cultivation periods. This system is designed to maintain optimal conditions for cell growth and productivity by ensuring a constant supply of nutrients and the removal of waste products. Cell perfusion is an alternative to traditional batch or fed-batch cell culture methods, offering advantages in terms of productivity, cell viability, and overall process control.

Key Components and Features of a Cell Perfusion System:

1. Bioreactor: The bioreactor is the vessel where cells are cultured. It is designed to support continuous perfusion and may include features such as a cell retention device.

2. Peristaltic Pumps: Peristaltic pumps are used to continuously circulate fresh media through the bioreactor. These pumps control the flow rate and maintain a steady supply of nutrients.

3. Media Reservoir: The system includes a reservoir where cell culture media is stored. The media reservoir is connected to the bioreactor, and the flow of media is regulated to achieve the desired perfusion rate.

4. Cell Retention Device: In some perfusion systems, a cell retention device is used to retain cells within the bioreactor while allowing the continuous flow of media. This ensures that cells are not washed out during the perfusion process.

5. Sensors: Various sensors, such as pH probes, dissolved oxygen sensors, and biomass sensors, monitor key parameters in the culture and provide real-time data for process control.

6. Control System: An automated control system adjusts and regulates parameters such as flow rates, nutrient concentrations, and temperature to maintain optimal conditions for cell growth.

7. Harvesting System: The system may include mechanisms for continuous or periodic harvesting of cells or cell products based on the specific requirements of the bioprocess.

Advantages of Cell Perfusion Systems:

1. Continuous Operation: Cell perfusion systems enable continuous operation, allowing for extended cultivation periods without the need for batch interruptions.

2. Increased Productivity: Continuous perfusion can lead to higher cell densities and enhanced productivity compared to traditional batch or fed-batch processes.

3. Improved Cell Viability: The constant supply of nutrients and removal of waste products contribute to improved cell viability and overall culture health.

4. Reduced Production Time: Continuous cell perfusion systems can reduce the overall production time for certain biopharmaceuticals.

Applications:

Cell perfusion systems are commonly used in various applications, including:

1. Biopharmaceutical Production: Production of therapeutic proteins, antibodies, and other biopharmaceuticals.

2. Vaccine Production: Cultivation of cells for vaccine production.

3. Enzyme Production: Continuous production of enzymes for industrial applications.

4. Cellular Agriculture: Cultivation of cells for the production of cultured meat or other cellular agriculture products.

Cell perfusion systems play a crucial role in optimizing cell culture processes, particularly in scenarios where prolonged cultivation is required, and maintaining optimal conditions is critical for achieving high yields of bioproducts.

Introduction:

Continuous biological production is a key step in improving drug quality and productivity. Improving the upstream process operation and perfusion culture is the key to increase the productivity of upstream biological process.

TOPGUN-PF intelligent perfusion regeneration system is a one-time system application for process development and production. It is designed and manufactured in full compliance with current pharmaceutical manufacturing

Code (CGMP) requirements, designed in accordance with ASME-BPE specifications, and adopts 3D modular design to bring customers a new use experience. The automatic control adopts PLC+PC, which can monitor and

record the pressure before and after the film, automatically adjust the flow rate of circulating liquid and filtered liquid in the system, record the curve of relevant process parameters, and query the historical record and trace back.

The products are widely used in cell perfusion culture, cell harvesting, plasmid fermentation, cell or lysate clarification and concentration, recombinant protein, virus and other purposes of product purification and concentration.

We provide various customized services (OEM&ODM) to global buyers, informing us of the requirements for bioprocess single use bags or other equipment, such as pipeline materials, length, diameter, clamp types, usage or photos of the products currently in use. You can also inquire with us based on the standard model numbers listed below.